Food industry waste - An opportunity for black soldier fly larvae protein production in Tanzania.

Black soldier fly larvae composting is an emerging treatment option with potential to improve biowaste valorization in cities of low-income countries. This study surveyed the current generation and management status of food industry biowaste and their availability and suitability as potential feedstock for black soldier fly larvae (BSFL) composting treatment in three Tanzania cities, Dar es Salaam, Mwanza, and Dodoma. Biowaste-generating food industry companies (n = 29) in the three cities were found to produce banana peels, mango seeds, sunflower press cake, brewery waste, and coffee husks in large quantities (~100,000-1,000,000 kg y-1). Around 50 % of these companies (16/29), primarily vegetable oil companies (10/11), either sold or gave away their waste as animal feed, while most companies (9/11) with unutilized food industry waste landfilled the generated biowaste. Multi-criteria analysis based on substrate availability criteria identified banana peels, mango seeds, and coffee husks with total score points of ≥10/12 as the most suitable feedstock for BSFL composting. However, multi-criteria analysis based on physical-chemical criteria identified brewery waste and sunflower press cake with total score points of ≥11/15 as the most suitable feedstock. Combined availability and physical-chemical properties of individual biowastes showed that all identified types of food industry biowaste can be suitable feedstock for producing BSFL biomass for protein production, but certain waste streams needed to be mixed with other waste streams prior to BSFL-composting to ensure sufficient availability and provide a balanced nutritional profile compared with the single-source biowastes. This study concluded that large volumes of food industry waste are being generated from food industry companies in Tanzania and there is need to establish new biowaste management interventions for resource recovery. Furthermore, for interested stakeholders in the waste management business, multi-stream BSFL-composting can be a suitable solution for managing and closing nutrient loops of the unutilized food industry biowaste in Tanzania and in other similar settings globally.

Process efficiency in relation to enzyme pre-treatment duration in black soldier fly larvae composting.

Black soldier fly larvae (BSFL) composting is a treatment in which biodegradable food waste is converted into animal-feed protein and organic fertiliser. BSFL composting has greatest potential for mixed food waste, but under European Union regulations only plant-based waste is permitted as feed for larvae. Biomass conversion efficiency (BCE) in BSFL composting is lower for plant-based waste than for mixed food waste. One way of improving BCE for plant-based waste is to add enzymes to make the waste more available to the larvae, but enzyme pre-treatment is not commonly applied prior to BSFL composting. Therefore this study examined the impact of enzyme pre-treatment duration on process efficiency in BSFL composting of lettuce-cabbage waste pre-treated with enzymes for 0-4 days. The results showed that total solids (TS) in larvae decreased with longer enzyme pre-treatment. Direct addition of enzymes at the start of BSFL treatment (0 day pre-treatment) resulted in 22% higher BCE on a volatile solids (VS) basis compared with the control, while longer pre-treatment did not improve BCE further. Much of the VS was respired in the 0-day pre-treatment, resulting in lower mass of residues at the end of treatment. Longer pre-treatment increased microbial respiration, suggesting that the microbial community consumed more easily available carbohydrates during the pre-treatment step, which counteracted the purpose of enzyme pre-treatment, i.e. increasing BCE during BSFL composting.

Inactivation of Cryptosporidium parvum oocysts and faecal indicator bacteria in cattle slurry by addition of ammonia.

AIMS: To determine inactivation of Cryptosporidium parvum oocysts and reduction of Escherichia coli and enterococci in cattle slurry added aqueous ammonia. METHODS AND RESULTS: Escherichia coli, enterococci and nonviable C. parvum oocysts (DAPI+PI+) were enumerated every second day for 2 weeks in cattle slurry amended with 60 mmol l-1 aq. ammonia and compared with untreated slurry at three temperatures. Regardless of temperature, the proportion of nonviable C. parvum oocysts increased significantly faster over time in slurry with added ammonia than raw slurry (P = 0·021) corresponding to 62·0% higher inactivation (P = 0·001) at day 14. Additionally, 91·8% fewer E. coli and 27·3% fewer enterococci were observed in slurry added ammonia at day 14 compared to raw slurry. CONCLUSION: The addition of aqueous ammonia to raw slurry significantly reduced the viability of C. parvum oocysts and numbers of bacterial indicators. Hence, ammonia is usable at lower pathogen concentrations in slurry before application to agricultural land. SIGNIFICANCE AND IMPACT OF THE STUDY: Livestock waste is a valuable source of plant nutrients and organic matter, but may contain high concentrations of pathogens like E. coli and Cryptosporidium sp. that can be spread in the environment, and cause disease outbreaks. However, die-off rates of pathogens in organic waste can increase following increasing ammonia concentrations.

Pre-treatment of banana peel to improve composting by black soldier fly (Hermetia illucens (L.), Diptera: Stratiomyidae) larvae.

Use of black soldier fly (Hermetia illucens (L.), Diptera: Stratiomyidae) larvae (BSFL) is among the solutions being explored to shift the value chain in organic waste management by producing valuable products. Although BSFL consume a range of substrates, nutrient-imbalanced materials with high hemicellulose and lignin content, e.g. manure and banana peel, yield low conversion into larval biomass. This study explored pre-treatment methods to improve the nutrient composition and digestibility of banana peel to achieve higher substrate conversion into BSFL biomass. The pre-treatment methods evaluated were microbial, chemical (non-protein nitrogen), heat-based, and combinations of these. All pre-treatments tested except heating resulted in more efficient BSFL conversion in terms of final larvae weight. The low BSFL responses in pre-treatments were caused by the observed high amounts of tannins and phenolic compounds mainly from the heating pre-treatment. Waste to biomass conversion ratio correlated negatively with substrate volatile solids (VS) and positively with the decrease in VS in pre-treatment. Microbial - 14 days pre-treatments provided the optimum pre-treatment time for the microorganisms to achieve maximum degradation of the substrates, facilitating larval assimilation of the released nutrients. Rhizopus oligosporus-14 days and ammonia + Rhizopus resulted in the most efficient BSFL treatment, measured as protein produced per kg incoming material.

Greenhouse gas emissions from small-scale fly larvae composting with Hermetia illucens.

Fly larvae composting is an emerging waste treatment alternative with great potential to increase revenue from food waste management. For wider implementation, fly larvae composting has to be evaluated in comparison with conventional systems, based on direct greenhouse gas (GHG) emission data for the treatment process, which are currently limited. This study evaluated direct emissions of CO2, CH4, N2O and NH3 from composting of food waste using black soldier fly (BSF) larvae (Hermetia illucens). Use of BSF larvae-associated bacteria in 7-day pre-treatment and seeding at larvae treatment start were evaluated and compared to larvae treatment without bacteria addition. The treatments were performed in a set of 14-day laboratory-scale experiments. Mean substrate reduction was 49 ±â€¯8% and bioconversion ratio was 24 ±â€¯8% (both dry matter basis). Direct GHG emissions from the fly larvae treatment process were generally very small, with emissions of CH4 and N2O equivalent to 0.38 kg CO2-equivalents per ton food waste treated assuming global warming potential over 100 years, while mean total CO2 emissions were 96 g CO2 per kg food waste treated. Additional emissions could be expected to occur in the pre-treatment process, which did not provide any significant improvement in bioconversion ratio or reduction in total GHG emissions during treatment. Similarly, use of BSF larvae-associated bacteria did not significantly improve process efficiency. No NH3 emissions were detected, as reflected in total N mass balance over the treatment cycle. The results show that total direct GHG emissions from food waste treatment by fly larvae composting are lower than those from conventional food waste treatment, and that pre-treatment and seeding of food waste with BSF larvae-associated bacteria do not further reduce total GHG emissions.

Decentralised black water treatment by combined auto-thermal aerobic digestion and ammonia - A pilot study optimising treatment capacity.

Partial heating of black water by auto-thermal aerobic digestion was combined with the addition of 1% w/w urea and monitoring of pathogens and indicator organisms over a 21-day period. After initial mixing, the 160 m3 black water (60 m3 heated and 100 m3 non-heated) was left undisturbed. The urea was confirmed to be fully degraded into ammonia (5.1 g N L-1) first after 14 days, while the pH stabilised at around 9.2 after one week. The initial temperature of 17 °C fell by 6 °C during the study. E. coli and Salmonella spp., which are sensitive to ammonia, were inactivated during the first few days of the study, despite the urea only being partly hydrolysed. At day 14, f-RNA bacteriophages could also no longer be detected. The more persistent somatic coliphages, Enterococcus spp. and Ascaris eggs, showed significant but slow inactivation. The treatment proved to be efficient with regards to salmonella, which is a target pathogen in the Swedish context, but for parasite egg inactivation a higher temperature was required. The treatment would benefit from more frequent stirring to speed up the hydrolysis of urea and thus improve treatment efficiency. The alternative treatment scheme could increase capacity by 2.4 times, albeit with a 40% higher cost per volume due to the increased use of urea.

Quality of greywater treated in biochar filter and risk assessment of gastroenteritis due to household exposure during maintenance and irrigation.

AIM: This study evaluated treatment of greywater (GW) by a biochar filter in Jordan and assessed the annual risks of infection (Pi-annual ), annual risk of disease (Pd-annual ) and disease burden (in disability-adjusted life years; DALYs) of gastroenteritis caused by Salmonella spp. and rotavirus due to ingestion of GW during system maintenance and consumption of green onions irrigated with treated and nontreated GW. METHODS AND RESULTS: The biochar filter efficiently removed 93% of biochemical oxygen demand (BOD5 ) and 85% of solids, while removal of Escherichia coli was insignificant. Treatment of GW decreased the median Pd-annual due to ingestion of GW from 1·39 × 10-2 to 6·0 × 10-3 for Salmonella spp. but did not affect Pd-annual caused by rotavirus (9·73 × 10-1 to 1·0). Consumption of onions irrigated with treated GW had a median Pd-annual of 1·25 × 10-9 to 1·2 × 10-8 for Salmonella spp. and 4·96 × 10-4 to 4·37 × 10-3 for rotavirus infection, which was 99·9 and 90% lower, respectively, than the risk when consuming onions irrigated with nontreated GW. The highest risks of gastrointestinal disease were thus associated mainly with direct ingestion of GW when maintaining the system. CONCLUSIONS: Garden produce irrigated with GW treated in biochar filter did not display intolerable risks of rotavirus-based gastroenteritis during summer season in the study area given that the produce is harvested 1 to 2 days, and washed, before consumption. SIGNIFICANCE AND IMPACT OF THE STUDY: This study contributes to scientific-based knowledge on the suitability of biochar filters for onsite greywater treatment and confirms the microbial safety of recycling treated greywater for garden irrigation.

Environmental impact from vermicomposting of organic waste in Kampala, Uganda.

Urban animal farming is becoming increasingly important in feeding the growing population of many sub-Saharan African cities. However, management of the animal manure generated is proving to be challenging due to space restrictions. Vermicomposting is one of the methods proposed to address this challenge. This study investigated the environmental performance of the vermicompost system by measuring the gaseous emissions generated from the system. In addition, the vermicompost system was compared with other manure management systems currently in use, using life cycle assessment (LCA) methodology. The emissions factors for the vermicompost system were found to be 10.8, 62.3 and 12.8 g/Megagram biowaste for methane, nitrous oxide and ammonia, respectively. LCA showed satisfactory performance of vermicomposting in terms of global warming and eutrophication potential, although if the vermicompost generated is dumped, this could lead to increased eutrophication. However, this is still much lower than the eutrophication caused by open dumping of untreated manure.

Fate of pharmaceuticals and pesticides in fly larvae composting.

A novel and efficient organic waste management strategy currently gaining great attention is fly larvae composting. High resource recovery efficiency can be achieved in this closed-looped system, but pharmaceuticals and pesticides in waste could potentially accumulate in every loop of the treatment system and spread to the environment. This study evaluated the fate of three pharmaceuticals (carbamazepine, roxithromycin, trimethoprim) and two pesticides (azoxystrobin, propiconazole) in a fly larvae composting system and in a control treatment with no larvae. It was found that the half-life of all five substances was shorter in the fly larvae compost (<10% of control) and no bioaccumulation was detected in the larvae. Fly larvae composting could thus impede the spread of pharmaceuticals and pesticides into the environment.

Modeling the inactivation of ascaris eggs as a function of ammonia concentration and temperature.

Ammonia sanitization is a promising technology for sanitizing human excreta intended for use as a fertilizer in agriculture. Ascaris eggs are the most persistent pathogens regarding ammonia inactivation and are commonly present in fecal sludge in low- and middle-income countries. In this study, a model for predicting ammonia inactivation of ascaris eggs was developed. Data from four previous studies were compiled and analyzed statistically, and a mathematical model for the treatment time required for inactivation was created. The inactivation rate increased with NH3 activity to the power of 0.7. The required treatment time was found to decrease 10-fold for each 16 °C temperature increase. Dry matter (DM) content and pH had no direct effect on inactivation, but had an indirect effect due to their impact on NH3 activity, which was estimated using the Pitzer approach. An additional model giving an approximation of Pitzer NH3 activity but based on the Emerson approach, DM content and total ammonia (NHTot) was also developed. The treatment time required for different log10 reductions of ascaris egg viability can thus easily be estimated by the model as a function of NH3 activity and temperature. The impact on treatment time by different treatment options can then be theoretically evaluated, promoting improvements of the treatment e.g. by adding urea or alkaline agents, or increasing the temperature by solar heating.

Reverse logistics system and recycling potential at a landfill: A case study from Kampala City.

The rapid growing population and high urbanisation rates in Sub-Saharan Africa has caused enormous pressure on collection services of the generated waste in the urban areas. This has put a burden on landfilling, which is the major waste disposal method. Waste reduction, re-use and recycling opportunities exist but are not fully utilized. The common items that are re-used and re-cycled are plastics, paper, aluminum, glass, steel, cardboard, and yard waste. This paper develops an overview of reverse logistics at Kiteezi landfill, the only officially recognised waste disposal facility for Kampala City. The paper analyses, in details the collection, re-processing, re-distribution and final markets of these products into a reversed supply chain network. Only 14% of the products at Kiteezi landfill are channeled into the reverse chain while 63% could be included in the distribution chain but are left out and disposed of while the remaining 23% is buried. This is because of the low processing power available, lack of market value, lack of knowledge and limited value addition activities to the products. This paper proposes possible strategies of efficient and effective reverse logistics development, applicable to Kampala City and other similar cities.

Simulation and verification of hydraulic properties and organic matter degradation in sand filters for greywater treatment.

To evaluate the treatment performance of vertical flow sand filters, the HYDRUS wetland module was used to simulate treatment in an experimental set-up. The laboratory filters were intermittently dosed with artificial greywater at a hydraulic loading rate of 0.032 m³ m⁻² day⁻¹ and an organic loading rate of 0.014 kg BOD5 m⁻² day⁻¹. The hydraulic properties of the filter were characterised, as were inflow and outflow concentrations of chemical oxygen demand (COD), biochemical oxygen demand (BOD), ammonia, nitrate and total nitrogen. The inverse simulation function of the HYDRUS software was used to calibrate the water flow model. The observed effect of water flowing faster along the column wall was included in the inverse simulations. The biokinetic model was calibrated by fitting heterotrophic biomass growth to measurements of potential respiration rate. Emphasis was put on simulating outflow concentrations of organic pollutants. The simulations were conducted using three models of varying degree of calibration effort and output accuracy. The effluent concentration was 245 mg COD L⁻¹ for the laboratory filters, 134 mg COD mg L⁻¹ for the model excluding wall flow effects and 338 mg COD mg L⁻¹ for the model including wall flow effects.

Treatment with Ca(OH)2 for inactivation of Salmonella Typhimurium and Enterococcus faecalis in soil contaminated with infected horse manure.

AIM: To investigate the inactivation of Salmonella enterica serovar Typhimurium and the faecal indicator Enterococcus faecalis in horse manure:soil mixtures by application of hydrated lime (Ca(OH)(2)). METHODS AND RESULTS: In laboratory incubations, the inhibitory effect of different concentrations of Ca(OH)(2), as well as different application techniques, was tested. Other variables were horse manure:soil ratio, incubation temperature (6 and 14°C) and soil type (sand/clay). Bacterial enumeration by the plate count method in samples taken at increasing intervals revealed that Ca(OH)(2) effectively reduced Salmonella Typhimurium numbers. However, to achieve a sufficient reduction, the Ca(OH)(2) had to be applied at a sufficient rate, and the amount required varied because of manure:soil ratio and incubation temperature. The results showed that a pH above 11 was needed and that a high pH had to be maintained for up to 7 days. An appropriate application technique for the Ca(OH)(2) was also important, so that a high pH was obtained throughout the whole material to be treated. In addition, a high manure:soil ratio in combination with a higher incubation temperature was found to rapidly neutralize the pH and to increase the risk of Salmonella re-growth. CONCLUSIONS: Application of Ca(OH)(2) can be an efficient method for treating a Salmonella-contaminated horse paddock. A high pH is a key factor in Salmonella inactivation, and thus, monitoring the pH during the treatment period is necessary. To avoid re-growth excess manure should be removed for separate treatment elsewhere. SIGNIFICANCE AND IMPACT OF THE STUDY: Persistence of Salmonella in horse paddocks poses a risk of disease transmission to healthy animals and people who come into contact with these animals. An efficient method to de-contaminate a Salmonella-contaminated soil would be a valuable tool for animal welfare and for public health.

Growth potential of faecal bacteria in simulated psychrophilic/mesophilic zones during composting of organic waste.

AIM: This study investigated the growth potential of Salmonella serotype Typhimurium and faecal indicator organisms in compost materials and the correlation between bacterial growth potential and the physico-chemical composition of the compost substrate and temperature. METHODS AND RESULTS: Survival of Salm. Typhimurium, Enterococcus spp. and total coliforms at 14, 24 and 37 degrees C was determined in material of different degrees of maturity collected from composting plants for household waste and manure. All three micro-organisms showed the potential for growth in the material from active composts (Solvita index 4) but inactivation generally occurred over time in mature compost material (Solvita index 7-8). CONCLUSIONS: Salm. Typhimurium had the potential for growth in psychrophilic/mesophilic (P/M) zones of immature compost material and its growth potential correlated negatively with the maturity of the compost and the temperature within the simulated P/M zone. SIGNIFICANCE AND IMPACT OF THE STUDY: The risk of pathogen regrowth in P/M zones during organic waste composting further emphasizes the importance of good management practices and of avoiding P/M zones in combination with low compost maturity.

Substrate composition and moisture in composting source-separated human faeces and food waste.

The composting of a faeces/ash mixture and food waste in relative proportions of 1:0, 1:1 and 1:3 was studied in three successive experiments conducted in Kampala, Uganda in 216 L reactors insulated with 75 mm styrofoam or not insulated. The faeces/ash mixture alone exceeded 50 degrees C for < or = 12 days in insulated reactors, but did not reach or maintain 50 degrees C in non-insulated reactors. Inclusion of food waste kept temperatures above 50 degrees C for over two weeks in insulated reactors except when the substrate was too wet. Escherichia coli and total coliform concentrations decreased below detection in material that exceeded 50 degrees C for at least six days. Enterococcus spp. decreased below detection in material that exceeded 50 degrees C for at least two weeks, but remained detectable after 1.5 months in material that exceeded 50 degrees C for less than two weeks, suggesting that a period of at least two weeks above 50 degrees C, combined with mixing, is needed to achieve sanitation. Initially substrates that were too wet proved a challenge to composting and ways of decreasing substrate moisture should be investigated. The results obtained are applicable to the management of small- to medium-scale composting of faeces/ash and food waste at household and institution levels, e.g. schools and restaurants.

Sanitation of faeces from source-separating dry toilets using urea.

AIM: To develop a reliable and simple method to produce safe fertilizers from human excreta using urea for sanitation of faeces. METHODS AND RESULTS: Urea was added to faecal matter (17% dry matter) at concentrations of 0.5-2% (w/w) and inactivation of Salmonella enterica subspecies 1 serovar Typhimurium (Salm. Typhimurium), Enterococcus spp. and the Salm. Typhimurium bacteriophage 28B was monitored at 14, 24 and 34 degrees C. Urea additions enhanced inactivation and inactivation rates were positively related to increasing NH(3) (aq) concentration and temperature. Salm. Typhimurium was the most sensitive of the organisms studied, while Enterococcus spp. showed more persistence, especially at lower temperatures. The bacteriophage was the most resistant organism studied. CONCLUSIONS: Salmonella reduction levels that meet requirements for safe reuse of faeces as fertilizer (i.e. 6 log(10) reduction) can be achieved for 1% urea within 2 months at 14 degrees C or within 1 week at 24 degrees C and 34 degrees C. SIGNIFICANCE AND IMPACT OF THE STUDY: The relationships between organism inactivation rates and temperature, ammonia and pH were identified. Urea treatment proved to be a robust and efficient option for safe recycling of plant nutrients.

Bench-scale composting of source-separated human faeces for sanitation.

In urine-diverting toilets, urine and faeces are collected separately so that nutrient content can be recycled unmixed. Faeces should be sanitized before use in agriculture fields due to the presence of possible enteric pathogens. Composting of human faeces with food waste was evaluated as a possible method for this treatment. Temperatures were monitored in three 78-L wooden compost reactors fed with faeces-to-food waste substrates (F:FW) in wet weight ratios of 1:0, 3:1 and 1:1, which were observed for approximately 20 days. To achieve temperatures higher than 15 degrees C above ambient, insulation was required for the reactors. Use of 25-mm thick styrofoam insulation around the entire exterior of the compost reactors and turning of the compost twice a week resulted in sanitizing temperatures (>or=50 degrees C) to be maintained for 8 days in the F:FW=1:1 compost and for 4 days in the F:FW=3:1 compost. In these composts, a reduction of >3 log(10) for E. coli and >4 log(10) for Enterococcus spp. was achieved. The F:FW=1:0 compost, which did not maintain >or=50 degrees C for a sufficiently long period, was not sanitized, as the counts of E. coli and Enterococcus spp. increased between days 11 and 15. This research provides useful information on the design and operation of family-size compost units for the treatment of source-separated faeces and starchy food residues, most likely available amongst the less affluent rural/urban society in Uganda.

In situ ammonia production as a sanitation agent during anaerobic digestion at mesophilic temperature.

AIM: To measure the sanitizing effect of mesophilic (37 degrees C) anaerobic digestion in high ammonia concentrations produced in situ. METHODS AND RESULTS: Indicator organisms and salmonella were transferred to small-scale anaerobic batch cultures and D-values were calculated. Batch cultures were started with material from two biogas processes operating at high (46 mmol l(-1)) and low (1.6 mmol l(-1)) ammonia concentration. D-values were shortened from c. 3 days to <1 day for the bacteria. MS2 had the same D-value (1.3 days) independent of ammonia concentration whereas PhiX174 and 28B were faster inactivated in the control (1.1 and 7.9 days) than in the high ammonia (8.9 and 39 days) batch cultures. CONCLUSION: Running biogas processes at high levels of ammonia shortens the time to meet EU regulation concerning reduction of salmonella and enterococci (5 log). Unless a minimum retention time of 2 days, post-treatment digestion is needed to achieve sufficient sanitation in continuous biogas processes. SIGNIFICANCE AND IMPACT OF THE STUDY: Running mesophilic biogas processes at high ammonia level produces residue with a high fertilizer value. With some stipulations concerning management parameters, such processes provide a method of bacterial sanitation without preceding pasteurization of the incoming organic waste.